Question

How is OD value calculated?

Answer 1

The easiest way to gauge how far along a culture is in its growth curve is by measuring the OD₆₀₀, or the optical density at 600 nm. This wavelength is specifically chosen for bacterial OD measurements because unlike UV wavelengths, 600 nm is not harmful to the culture.May 29, 2018

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For E. coli cell cultures, OD600 of 1.0 = 8 x 10⁸cells/ml. E. coli concentration can be calculated by multiply OD600 readings with 8 x 10⁸.Apr 1, 2019

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For absorbance measurements, the optical density (O.D.) is a logarithmic measurement of the percent transmission (%T) and it can be represented by the equation, A = log10 100 / %T. That means a sample with: 1 O.D. allows 10% of light to be transmitted through the sample.